Methods of treating skin disease, scalp disease, sensitive skin or suppressing hair loss with microbubble washing compositions

ABSTRACT

The present invention provides a microbubble washing composition for washing a human body or an animal, a microbubble washing method using the microbubble washing composition, and a microbubble washing apparatus suitable for carrying out the method are provided which are capable of safely removing sebum and old keratin adhering to the surfaces of the pores in a short period of time, keeping the skin clean for a long period of time, preventing an odor. Specifically, the present invention provides a microbubble washing composition for washing a human body or an animal including a protease and a lipase, a microbubble washing method for washing a human body or an animal including washing a human body or an animal using a washing liquid containing the washing composition and microbubbles, and a microbubble washing apparatus.

CROSS REFERENCE TO RELATED APPLICATIONS

The present application is a Divisional of co-pending U.S. applicationSer. No. 11/826,989, filed on Jul. 19, 2007, and for which priority isclaimed under 35 U.S.C. § 120; and this application claims priority ofJapanese Application No. 2006-274959, filed on Oct. 6, 2006 under 35U.S.C. § 119. U.S. application Ser. No. 11/826,989 and JapaneseApplication No. 2006-274959 are incorporated by reference in theirentireties.

BACKGROUND OF THE INVENTION

1. Field of the Invention

The present invention relates to a microbubble washing composition forwashing a human body or an animal, a microbubble washing method usingthe microbubble washing composition, and a microbubble washingapparatus.

2. Description of Related Art

A washing agent containing a surfactant and the like has been used towash human hair or skin or a pet such as a dog.

However, since a known washing agent cannot reliably remove sebum andold keratin adhering to the surfaces of the pores in a short period oftime, dandruff, itching, fallen hair, odor, and the like tend to occurafter washing. This makes it difficult to keep the skin clean for a longperiod of time. Moreover, when washing a person or an animal (pet)suffering from skin disease or having a sensitive skin using a knownwashing agent or the like, the skin disease gets worse or the skinbecomes rough, for example. In recent years, an increased number ofpeople keep a pet such as a dog indoors. Therefore, prevention of skindisease, odor, fallen hair, and the like of the pet has beenincreasingly demanded.

As related-art technology, JP-A-2-227500 discloses an enzyme-basedliquid washing composition containing a nonionic washing compound, alipase, a lower fatty alcohol, a water-soluble salt of a lower aliphaticcarboxylic acid, and a protease in a specific ratio.

JP-A-2-69600 discloses a tablet-type foaming washing agent for washing anursing bottle which is prepared by adding a specific surfactant to acatabolic enzyme (e.g. protease or lipase) which decomposes milk cakeremaining in a nursing bottle, mixing a foaming agent containing sodiumhydrogen carbonate and citric anhydride with the mixture, adding anexcipient and the like to the mixture, and forming the mixture into atablet.

JP-A-2006-34473 discloses a tableware washing/drying method whichincludes a washing step of immersing used tableware in a bath of anaqueous washing medium containing an amylolytic enzyme, a protease, anda lipase and applying vibration to the aqueous washing medium.

JP-T-2004-534528 discloses a preparation containing a specific proteaseand other enzymes such as protease other than the specific protease,amylase, cellulase, hemicellulase, and/or lipase.

However, the washing agent, the washing method, and the preparationdisclosed in the above documents are mainly aimed at washing tableware,clothes, and the like, and are not used for washing a human body or ananimal.

SUMMARY OF THE INVENTION

The present invention has been achieved in view of the above-describedsituation of the related art. An object of the present invention is toprovide a microbubble washing composition for washing a human body or ananimal, a microbubble washing method using the microbubble washingcomposition, and a microbubble washing apparatus suitable for carryingout the method which can reliably remove sebum and old keratin adheringto the surfaces of the pores of a human body or an animal in a shortperiod of time and keep the skin clean for a long period of time.

The inventors of the present invention have conducted extensive studiesin order to achieve the above object. As a result, the inventors havefound that sebum and old keratin adhering to the surfaces of the poresof a human body or an animal and wastes (sebum) inside the pores can bereliably removed in a short period of time so that the skin can be keptclean for a long period of time, skin disease can be prevented, and thehealing of skin disease can be accelerated by using a washing liquidobtained by adding a microbubble washing composition including aprotease and a lipase to water and introducing microbubbles into thecomposition when washing human hair or skin or a pet such as a dog. Thepresent invention has been achieved based on the above finding.

A first aspect of the present invention provides a microbubble washingcomposition according to (1) to (7).

(1) A microbubble washing composition for washing a human body or ananimal, the composition comprising a protease and a lipase.(2) The microbubble washing composition according to (1), wherein thecomposition contains the protease in an amount of 0.01 to 0.5 wt % basedon the total amount of the composition, and contains the lipase in anamount of 0.1 to 1.0 wt % based on the total amount of the composition.(3) The microbubble washing composition according to (1) or (2), furthercomprising an alkali metal salt.(4) The microbubble washing composition according to (3), wherein thealkali metal salt is at least one compound selected from the groupconsisting of an alkali metal carbonate, an alkali metal hydrogencarbonate, an alkali metal sulfate, an alkali halide, and an alkalimetal salt of boron.(5) The microbubble washing composition according to (3) or (4), whereinthe composition contains the alkali metal salt in an amount of 80 to99.5 wt % based on the total amount of the composition.(6) The microbubble washing composition according to any one of (1) or(5), further comprising an silica-based desiccant.(7) The microbubble washing composition according to (6), wherein thecomposition contains the silica-based desiccant in an amount of 0.01 to1 wt % based on the total amount of the composition.

According to a second aspect of the present invention, a microbubblewashing method according to (8) is provided.

(8) A microbubble washing method for washing a human body or an animal,the method comprising washing a human body or an animal using a washingliquid comprising the microbubble washing composition according any oneof (1) to (7) and microbubbles.

According to a third aspect of the present invention, a microbubblewashing apparatus according to (9) and (10) is provided.

(9) A microbubble washing apparatus for washing a human body or ananimal, the apparatus comprising a discharge section which discharges awashing liquid comprising the microbubble washing composition accordingany one of (1) to (7), wherein microbubbles are introduced into thewashing liquid.(10) A microbubble washing apparatus for washing a human body or ananimal, the apparatus comprising a washing bath in which a washingliquid comprising the microbubble washing composition according any oneof (1) to (7) is stored, and a discharge section which discharges wateror the washing liquid comprising microbubbles.

According to the present invention, a microbubble washing compositionfor washing a human body or an animal can be provided which can reliablyremove sebum and old keratin adhering to the surfaces of the pores in ashort period of time and keep the skin clean for a long period of timewhen used in combination with microbubbles.

Since the microbubble washing composition according to the presentinvention does not damage skin, hair, and the like due to low irritatingproperties, the microbubble washing composition according to the presentinvention can be safely used for a person having a sensitive skin, anelderly person having a weak skin, or a human or an animal (pet)suffering from skin disease.

The microbubble washing method according to the present invention canreliably remove the cause of an allergy, fallen hair, dandruff, itching,and the like in a short period of time to keep the skin clean for a longperiod of time, prevent skin disease, and prevent odor for a long periodof time by combining the microbubble washing composition according tothe present invention and the microbubbles.

Applying the microbubble washing method according to the presentinvention to a person or an animal suffering from skin disease can keepthe skin clean for a long period of time to improve the efficacy of amedicine applied after washing.

Since the microbubble washing method according to the present inventionis convenient and safe, the microbubble washing method according to thepresent invention may be suitably used to wash human hair, a pet, or thebody of a person requiring nursing care in the home or in nursingfacilities in the same manner as a known washing method.

A significantly excellent washing effect can be obtained by introducingthe microbubbles into the microbubble washing composition according tothe present invention in comparison with the case of washing using onlythe microbubbles.

Specifically, the present invention exhibits an effect of reliablyremoving sebum and old keratin adhering to the surfaces of the pores ofa human body or an animal and wastes (sebum) inside the pores in a shortperiod of time and keeping the skin clean for a long period of time, aneffect of preventing skin disease, and an effect of accelerating thehealing of skin disease.

When washing a pet (animal) such as a dog using the microbubble washingmethod according to the present invention, the coat of the pet becomessoft.

The microbubble washing apparatus according to the present invention maybe suitably used to carry out the microbubble washing method accordingto the present invention.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1 is a view showing the configuration of an example of a device forcarrying out a method of washing a dog according to the presentinvention.

FIG. 2 is a view showing the configuration of a main body 1.

DETAILED DESCRIPTION OF THE INVENTION AND PREFERRED EMBODIMENTS

The present invention is described below in detail in the order of 1) amicrobubble washing composition, 2) a microbubble washing method, and 3)a microbubble washing apparatus.

1) Microbubble Washing Composition

A first aspect of the present invention relates to a microbubble washingcomposition for washing a human body or an animal, the compositioncomprising a protease and a lipase.

The protease used in the microbubble washing composition according tothe present invention (hereinafter may be called “composition accordingto the present invention”) refers to a generic name of enzymescatalyzing hydrolysis of peptide bonds.

The protease has a specific optimum pH, and may be classified as anacidic proteinase, a neutral proteinase, or an alkaline proteinase. Inthe present invention, any of these proteases may be used withoutspecific limitations.

As non-limiting examples of the protease, chymotrypsin, subtilisin,pepsin, cathepsin D, thermolysin, papain, caspase, bromelain, actinidin,ficin, trypsin, pancreatin, and the like can be given.

These proteases may be used either individually or in combination of twoor more.

As specific examples of the protease, Protin AC 10F (manufactured byDaiwa Kasei K.K.), Savinase, Alcalase, Esperase, Durazyme (manufacturedby Novozymes), Maxapem, Maxatase (manufactured by Gist-brocades),Bioplaze (manufactured by Nagase Biochemicals, Ltd.), and the like canbe given.

It is preferable to use at least pancreatin in order to obtain anexcellent washing effect. It is more preferable to use pancreatin andother proteases in combination.

The amount of protease used is not particularly limited. The protease ispreferably used in an amount of 0.01 to 0.5 wt %, and more preferably0.2 to 0.3 wt % based on the total amount of the composition.

The lipase used in the composition according to the present invention isan enzyme which hydrolyzes fats into glycerol and a fatty acid.

As non-limiting examples of the lipase, a lipase from Rhizopus arrhizus,a lipase from Aspergillus niger, and a lipase from a mold such asRhizopus delemar; a lipase from yeast such as Candida cylindracea; alipase from a bacteria such as Pseudomonas; a lipase which exists in thedigestive tract tissue of a ruminant during a lactation period such aspregastric lipase or oral lipase; pig liver lipase; and the like can begiven.

These lipases may be used either individually or in combination of twoor more. It is preferable to use two or more types of lipases in orderto obtain a more excellent washing effect.

Specific examples of the lipase include Sumizyme NLS (manufactured byShin-Nihon Kagaku Kogyo, Co., Ltd.), Lipase M “Amano” 10, Lipase M“Amano” 10, Lipase G “Amano” 50, Lipase F-AP15, Lipase AY “Amano” 30G,Lipase R “Amano” G, Lipase T “Amano”, Lipase MER “Amano” (manufacturedby Amano Enzyme Inc.), Picantase R8000, Picantase A (manufactured byRobin), Toyozyme and LIP (manufactured by Toyobo Co., Ltd.), LilipaseA-10FG, Lilipase AF-5 (manufactured by Nagase ChemteX Corporation),Grindamyl EXEL639 (manufactured by Danisco Culter Japan), Clear-LensLipo, Liporaze, Lipex, Lipozyme, Resinase, Paratase, Greasex, Lipopan,Novozyme 435, Lecitase (manufactured by Novozymes), Lipase MY, LipaseOF, Lipase PL, Lipase QLM, Lipase AL, Phospholipase D (manufactured byMeito Sangyo Co., Ltd.), Enzylon AKG (manufactured by Rakuto KaseiIndustrial Co., Ltd.), Phospholipase A1 (manufactured by Sankyo LifetechCo., Ltd.), Lipomod 699L, Lysomax PF (manufactured by Genencor KyowaCo., Ltd.), and the like.

The lipase used is usually used in an amount of 0.1 to 1.0 wt %, andpreferably 0.3 to 0.8 wt % based on the total amount of the composition.

It is preferable that the washing composition according to the presentinvention further include an alkali metal salt in addition to theprotease and the lipase.

The alkali metal salt improves the washing effect, softens water (warmwater), and functions as a filler.

The alkali metal salt used is not particularly limited. Examples of thealkali metal salt include alkali metal carbonates such as sodiumcarbonate and potassium carbonate; alkali metal hydrogen carbonates suchas sodium hydrogen carbonate and potassium hydrogen carbonate; alkalimetal sulfates such as sodium sulfate and potassium sulfate; alkalihalides such as sodium chloride and potassium chloride; alkali metalsalts of boron such as sodium tetraborate (borax) and sodium borate;alkali metal silicates such as sodium silicate; alkali metal sulfidessuch as sodium sulfide; alkali metal nitrates such as sodium nitrate;alkali metal phosphates such as sodium phosphate and sodium dihydrogenphosphate; thiosulfates of an alkali metal such as sodium thiosulfate;and the like.

These alkali metal salts may be used either individually or incombination of two or more.

It is preferable that the composition according to the present inventioninclude at least one alkali metal salt selected from the groupconsisting of the alkali metal carbonates, the alkali metal hydrogencarbonates, the alkali metal sulfates, the alkali halides, and thealkali metal salts of boron. It is more preferable that the compositioninclude at least one alkali metal salt selected from the groupconsisting of sodium hydrogen carbonate, sodium sulfate, and borax. Itis particularly preferable that the composition include sodium hydrogencarbonate, sodium sulfate, and borax.

The alkali metal salt is usually used in an amount of 80 to 99.5 wt %,and preferably 95 to 99 wt %.

When the washing composition according to the present invention includessodium hydrogen carbonate, sodium sulfate, and borax, sodium hydrogencarbonate is preferably used in an amount of 10 to 35 wt %, andparticularly preferably 20 to 30 wt % based on the total amount of thecomposition, sodium sulfate is preferably used in an amount of 30 to 70wt %, and particularly preferably 40 to 60 wt % based on the totalamount of the composition, and borax is preferably used in an amount of10 to 35 wt %, and particularly preferably 20 to 30 wt % based on thetotal amount of the composition.

It is preferable that the composition according to the present inventionfurther include a silica-based desiccant.

The desiccant stabilizes the potencies of the protease and the lipase.As examples of the silica-based desiccant, Sylysia (porous fine silicapowder) and the like can be given.

The silica-based desiccant is usually used in an amount of 0.01 to 1 wt%, and preferably 0.1 to 0.2 wt %.

If necessary, other components may be further added to the compositionof the present invention.

As examples of other components, inorganic salts other than theabove-mentioned alkali metal salts, a perfume, a coloring agent, anantiseptic agent, an antibacterial agent, a tackiness agent, atherapeutic component, and other additives usually used in a washingagent can be given.

As examples of the inorganic salts other than the alkali metal salts,magnesium salts such as magnesium carbonate and magnesium sulfate;calcium salts such as calcium carbonate, calcium nitrate, calciumthiosulfate, and calcium hydrogen phosphate; alum; and the like can begiven.

The amount of inorganic salts used may be appropriately determinedinsofar as the effects of the microbubble washing composition accordingto the present invention are not impaired.

Examples of the perfume include essential oils such as abies oil,angelica oil, anisie oil, copaiba balsam, basil oil, bay oil, bergamotoil, birch oil, rosewood oil, cajabute oil, cassia oil, acacia oil,cedar wood oil, chamomile oil, cinnamon oil, cinnamon leaf oil,citronella oil, elemi oil, eucalyptus oil, geranium oil, white-cedarleaf oil, cypress oil, lavandin oil, lavender oil, lemon oil, mint oil,neroli oil, nutmeg oil, oakmoss oil, ocotea oil, patchouli oil,palmarosa oil, plai oil, rose oil, rosemary oil, sandalwood oil, vetiveroil, and ylang ylang oil; synthetic perfumes such as synthetic musk suchas limonene, terpinolene, p-cymene, 9-decenol, mugol, myrcenol, bomeol,vetiverol, t-butylcyclohexanol, anisole, anethole, safrole, citral,citronellal, cinnamaldehyde, anise aldehyde, cyclamen aldehyde, citral,acetophenone, benzophenone, acetonaphthone, nerone, and nitromusk,geranyl acetate, bomyl acetate, phenylethyl acetate, myrcenyl acetate,methyl benzoate, methyl cinnamate, methyl anthranilate, syntheticoakmoss, and coumarin; and the like.

The perfume is usually used in an amount of 0 to 0.5 wt %, andpreferably 0.1 to 0.3 wt %.

As the coloring agent, a dye, a natural pigment, an inorganic dye, andthe like may be used.

Examples of the dye include Red No. 2, Red No. 3, Red No. 102, Red No.104-1, Red No. 105-1, Red No. 106, Red No. 213, Red No. 227, Red No.230-1, Yellow No. 4, Yellow No. 5, Yellow No. 201, Yellow No. 201-1,Yellow No. 203, Orange No. 205, Green No. 3, Green No. 201, Green No.204, Blue No. 1, Blue No. 2, Blue No. 205, Violet No. 201, Brown No.201, and the like.

Examples of the natural pigment include a chlorophyll, a gardenia, aflavonoid, a carotinoide, a quinone, and a riboflavin.

Examples of the inorganic dye include titanium dioxide, talc, kaolin,mica, magnesium silicate, silicic acid anhydride, calcium carbonate,magnesium carbonate, and the like.

These coloring agents may be used either individually or in combinationof two or more.

The coloring agent is usually used in an amount of 0 to 0.5 wt %, andpreferably 0.1 to 0.2 wt %.

Examples of the antiseptic agent include sodium benzoate, methylp-hydroxybenzoate, ethyl p-hydroxybenzoate, propyl p-hydroxybenzoate,butyl p-hydroxybenzoate, isobutyl p-hydroxybenzoate, benzyl alcohol,benzalkonium chloride, and the like.

Examples of the tackiness agent include hydroxyethylcellulose,hydroxypropylmethylcellulose, methylcellulose, xanthan gum, polyethyleneglycol distearate, polyethylene glycol monostearate, and the like.

The method of producing the composition according to the presentinvention is not particularly limited. For example, a method may beemployed in which a mixture of the protease, the lipase, and otheroptional components is uniformly mixed and stirred using a stirrer suchas a Vert-o-Mix, a Nauta mixer, a universal mixer/stirrer, a ribbonmixer, a V-shaped mixer, or the like to prepare a powder.

The composition according to the present invention can reliably removesebum and old keratin adhering to the surfaces of the pores of a humanbody (hair or skin) or an animal in a short period of time and keep theskin clean for a long period of time.

Since the composition according to the present invention does not damagethe skin, hair, and the like due to low irritating properties, thecomposition according to the present invention can be safely used for aperson having a sensitive skin, an elderly person having a weak skin, ora human or an animal suffering from skin disease.

2) Microbubble Washing Method

A second aspect of the present invention relates to a microbubblewashing method for washing a human body or an animal, the methodcomprising washing a human body or an animal using a washing liquidcomprising the composition according to the present invention andmicrobubbles.

Specifically, the microbubble washing method according to the presentinvention includes adding an appropriate amount of the compositionaccording to the present invention to clean water to prepare a washingliquid, introducing microbubbles into the washing liquid, and washing ahuman body or an animal using the resulting washing liquid.

More specifically, the microbubble washing method according to thepresent invention includes adding an appropriate amount of thecomposition according to the present invention to clean water to preparea washing liquid, introducing microbubbles into the washing liquid, andwashing human hair, a human body (skin or foot bottom), or an animal(e.g. dog) using the resulting washing liquid.

When washing human hair, the composition according to the presentinvention is used to prepare the washing liquid in an amount of usually1 to 10 g, and preferably 3 to 7 g per 6 to 10 liters of water. Whenwashing human skin or foot bottom, the composition according to thepresent invention is used to prepare the washing liquid in an amount ofusually 1 to 10 g, and preferably 3 to 7 g per 6 to 10 liters of water.When washing an animal such as a dog, the composition according to thepresent invention is used to prepare the washing liquid in an amount ofusually 1 to 10 g, and preferably 3 to 7 g per 6 to 10 liters of water.

The microbubble washing time is usually about one minute to several tensof minutes, although the time varies depending on the type andconcentration of the microbubble washing composition, the washingportion, and the like.

The washing temperature is not particularly limited. The washingtemperature is preferably 20 to 40° C. from the viewpoint of anexcellent washing effect and relaxation.

Since the microbubble washing method according to the present inventioncan reliably remove sebum and old keratin adhering to the surfaces ofthe pores in a short period of time and keep the skin clean for a longperiod of time, skin disease and the like can be prevented, and thecause of an allergy, fallen hair, dandruff, itching, and the like can beremoved. Moreover, odor can be suppressed for a long period of time.

The microbubble washing method according to the present invention cankeep the skin clean to improve the efficacy of a medicine or the likeapplied after washing, thereby accelerating the healing of skin disease.Moreover, the microbubble washing method according to the presentinvention may be suitably used to wash a person who cannot be frequentlywashed, such as a person requiring nursing care.

The microbubble washing method according to the present inventionexhibits a remarkably excellent washing effect in comparison with thecase of washing a human body or an animal using water (or warm water)containing only the composition according to the present invention orwater (or warm water) containing only microbubbles.

Specifically, the microbubble washing method according to the presentinvention ensures that the composition according to the presentinvention and the microbubbles exhibit the effect of reliably removingsebum and old keratin adhering to the surfaces of the pores of a humanbody or an animal and wastes (sebum) inside the pores in a short periodof time and keeping the skin clean for a long period of time, the effectof preventing skin disease, and the effect of accelerating the healingof skin disease. Since the washing liquid containing the microbubblesdoes not adversely affect the cells of a human body or an animal, doesnot damage the skin, hair, and the like of a human or an animal, and isenvironmentally friendly, the washing liquid can be used safely.

The microbubbles are minute bubbles with a diameter of 50 μm or less,and are reduced in size and disappear (completely dissolve) in water,differing from normal bubbles. The microbubbles carry negative ions anddisappear in water while bonding to dirt (positive ions) and floating,thereby causing the dirt to float. When using the washing liquidcontaining the microbubbles to wash a human body or an animal, minutemicrobubbles can reach the interior of the pores to adsorb and removewastes.

In order to obtain the washing liquid containing the microbubbles, amicrobubble generation device described later is usually used. Sincewater has a high surface tension, it is impossible to produce bubbleswith a diameter of 100 μm or less by normal bubbling.

The microbubble generation device is not particularly limited insofar asthe device can produce microbubbles. A known microbubble generationdevice may be used. Examples of the microbubble generation deviceinclude a device which produces microbubbles in a venturi tube (tubewith a narrow constriction) or a shower head including an orifice plate(doughnut plate with a center opening) (e.g. JP-A-2006-116518 andJapanese Patent Application No. 2006-77553); a device which producesmicrobubbles by injecting a liquid mixed with a gas into a main pipedisposed in a liquid and causing the liquid to collide with a collisionwall disposed downstream of main pipe (JP-A-2005-334869); a device whichproduces microbubbles by injecting pressurized air into water through amesh member or a porous plate with a minute pore size using apressurized air supply source such as an air pump (JP-A-2006-68631); adevice which produces microbubbles by producing a whirling water streamand shearing air using the water stream (JP-A-2003-126665); and thelike. When washing human hair, it is preferable to use the microbubblegeneration device disclosed in Japanese Patent Application No.2006-77553.

Examples of the method of washing a human body or an animal using thewashing liquid containing the microbubbles include (i) a method in whicha washing liquid obtained by adding an appropriate amount of thecomposition according to the present invention to clean water (or warmwater) is placed in a container such as a tank, the washing liquid isintroduced into the microbubble generation device using an appropriatemethod, microbubbles are introduced into the washing liquid in thedevice, and a human body or an animal is washed by discharging thewashing liquid containing the microbubbles from a shower nozzle, (ii) amethod in which clean water (or warm water) is placed in a washing bath,an appropriate amount of the composition according to the presentinvention is added to the washing bath to prepare a washing liquid,water (or warm water) containing microbubbles is introduced into thewashing liquid from a nozzle connected to the microbubble generationdevice to prepare a washing liquid containing microbubbles, and a humanbody or an animal is washed using the resulting washing liquid; and thelike. In the present invention, these methods may be used incombination.

When using the method (i), it is preferable to circulate the washingliquid while removing contaminants and the like through a filter inorder to efficiently utilize the washing liquid.

3) Washing Apparatus

A third aspect of the present invention relates to a washing apparatusfor washing a human body or an animal comprising a discharge sectionwhich discharges a washing liquid containing the composition accordingto the present invention, wherein microbubbles are introduced into thewashing liquid, or a washing apparatus for washing a human body or ananimal comprising a washing bath in which a washing liquid containingthe composition according to the present invention is stored, and adischarge section which discharges water or the washing liquidcontaining microbubbles.

Examples of the specific configuration of the washing apparatusaccording to the present invention include (□) a washing apparatuscomprising a storage section in which a washing liquid obtained byadding the composition according to the present invention is stored, amicrobubble generation section which introduces microbubbles into thewashing liquid supplied from the storage section, and a shower nozzlethrough which the washing liquid containing the microbubbles isdischarged, (□) a washing apparatus comprising a washing bath in which awashing liquid obtained by adding the composition according to thepresent invention is stored, a nozzle section which supplies water (orwarm water) containing microbubbles to the washing liquid stored in thewashing bath to prepare the washing liquid containing the microbubbles,and a microbubble generation section which supplies the water (or warmwater) containing the microbubbles to the nozzle section; and the like.

Examples of the microbubble generation section of the washing apparatusaccording to the present invention include the microbubble generationdevices given as examples of the microbubble generation devices whichmay be used to obtain the washing liquid containing the microbubbles inthe washing method according to the present invention.

Specific examples are described below which wash a human or a pet(animal) using the washing apparatus according to the present invention.

A first specific example is an example of washing a dog (pet).

FIG. 1 is schematically shows a washing apparatus used to carry out thisspecific example. The washing apparatus shown in FIG. 1 includes awashing bath 20 in which a dog to be washed is placed, and a microbubblegeneration section 10.

FIG. 2 is an enlarged view of the microbubble generation section 10. Themicrobubble generation section 10 shown in FIG. 2 includes a main body1, a suction pipe 4 through which a washing liquid is transported fromthe washing bath 20 to the main body 1, a filter unit 5 which removesdirt and the like from the washing liquid, a screw pump 12 which pumpsthe washing liquid and air, an air filter 14 connected to an inlet port12 a of the screw pump 12 through a check valve 13, a gas-liquidseparator 15 which mainly discharges unnecessary air to the outside, adischarge pipe 2 through which the washing liquid is transported fromthe main body 1 to the washing bath 20, a nozzle unit 7 including anorifice plate for producing microbubbles, and a shower head 8 which isdisposed on the end of the discharge pipe 2 and through which thewashing liquid containing microbubbles is discharged.

A heater unit may be provided in an appropriate place in the main body 1of the microbubble generation section 10 or the washing bath 20, and thetemperature of the washing liquid in the washing bath 20 or the washingliquid discharged from the shower head 8 may be controlled at apredetermined suitable temperature (e.g. about 40° C.) by heating thewashing liquid using the heater unit.

First, warm water at about 38° C. is placed in the washing bath 20. Theamount of warm water is adjusted so that the body (portion below thehead) of the dog is immersed in the warm water in the washing bath, asshown in FIG. 1. The composition according to the present invention isadded to the warm water in an amount of 5 to 6 g per 5 to 10 liters ofthe warm water in the washing bath, and the mixture is lightly stirred.

The washing composition used in this example is prepared by mixingborax: 25 wt %, sodium hydrogen carbonate: 25 wt %, sodium sulfate: 50wt %, pancreatin: 0.1 wt %, Protin AC: 0.15 wt %, lipase: 0.5 wt %,Sylysia: 0.15 wt %, perfume: 0.2 wt %, and dye: 0.12 wt % using astirrer.

The end of the suction pipe 4 is placed at the bottom of the washingbath 20. The washing liquid in the washing bath 20 is introduced intothe screw pump 12 from the suction pipe 4 through the filter unit 5 byoperating the screw pump 12. An appropriate amount of air is pumped intothe screw pump 12 through the air filter 14.

In the screw pump 12 into which the washing liquid and the air areintroduced, bubbles are shorn and reduced in size by an impeller whichis rotated in the screw pump 12, whereby air is dissolved in the washingliquid. The washing liquid in which air is dissolved is discharged fromthe outlet port 12 b of the screw pump 12 together with undissolvedbubbles and supplied to the gas-liquid separator 15. In the gas-liquidseparator 15, dissolution of air in the washing liquid is promoted, andundissolved air is discharged to the atmosphere through a relief valve15 a provided at the upper portion of the gas-liquid separator 15.

The washing liquid in which a large amount of air is dissolved passesthrough the discharge pipe 2 from the gas-liquid separator 15, isdischarged from the shower head 8 provided on the end of the dischargepipe 2 placed at the bottom of the side surface of the washing bath 20,and is returned to the washing bath 20.

The nozzle unit 7 is attached to the shower head 8. A number of orificeplates are provided in the nozzle unit 7 in series. The washing liquidis compressed and expanded each time the washing liquid passes throughthe orifice plate, and air dissolved in the washing liquid producemicrobubbles in the washing liquid. The washing liquid containing themicrobubbles is discharged into the washing bath from the shower head 8.

The washing bath, into which the washing liquid containing themicrobubbles discharged, is filled with minute bubbles and becomesclouded. Since the microbubbles have a small buoyancy, the microbubblesfloat in the water for a long period of time without immediatelyreaching the water surface, whereby the effects are maintained.

After the washing liquid in the washing bath 20 is filled with themicrobubbles, the operation of the screw pump 12 may be stopped, or thescrew pump 12 may be continuously operated. The dog is bathed in thewashing bath 20 for about 5 to 15 minutes to wash the body of the dog.

The head of the dog which is not immersed in the washing liquid in thewashing bath 20 may be washed by lifting the shower head 8 on the end ofthe discharge pipe 2 placed at the bottom of the side surface of thewashing bath 20 and discharging the washing liquid containing themicrobubbles from the shower head 8 onto the dog's head, for example.

When the washing liquid containing the microbubbles contacts the body ofthe dog, the protease and the lipase in the washing compositionaccording to the present invention placed in the washing batheffectively decompose and remove the sebum and old keratin adhering tothe surfaces of the pores of the dog, whereby the surface of the body ofthe dog is washed. Since the microbubbles can easily enter the interiorof the pores after the dirt adhering to the surfaces of the pores isdecomposed and removed, wastes (sebum) and the like inside the pores canbe adsorbed due to the surface tension and effectively removed.

The microbubbles contained in the washing liquid break on the surface ofthe body of the dog to produce pulse impact, and the dirt and the likeadhering to the body of the dog can be effectively removed by themicrovibration.

As described above, since a person doing the washing need not scrub thedog's skin using a sponge or the like, wastes inside the pores can bereliably removed in a short period of time without damaging the dog'sskin and hair.

After the completion of washing, the washing composition is rinsed awayby spraying water (or warm water) on the body of the dog from a normalshower nozzle or the like.

When washing the dog using the washing apparatus according to thepresent invention, positive effects, such as (a) the coat becomes soft,(b) the skin becomes pink, (c) animal odor is reduced, (d) skin troublessuch as an allergy, dandruff, and itching are reduced, (e) fallen hairis reduced, and (f) the skin can be kept clean for a long period of timecan be obtained, in comparison with the case of washing the dog using aknown pet washing agent.

In particular, the period of time until odor occurs after washing isnearly doubled. Moreover, since the skin can be kept clean for a longperiod of time after washing, the present invention is effective forpreventing and healing skin disease. Since the present invention usesthe washing liquid including the composition according to the presentinvention and the microbubbles, skin irritation occurs to only a smallextent. Moreover, the washing time is reduced, and the washing agent iseasily rinsed away. Therefore, the burden on the dog (animal) isreduced.

An example of a method of washing human hair is described below as asecond specific example.

This method uses an apparatus similar to the washing apparatus used inthe method of washing the dog except that the washing bath 20 isomitted, the discharge pipe 2 and the suction pipe 4 are connected to awashing cap which can be fitted onto the human head in the same manneras a cap, and a cartridge tank (storage section) is provided in the mainbody 1.

The washing cap is fitted onto the human head so that the entire hair ispositioned in the space formed when fitting the washing cap, and has astructure in which the washing liquid discharged into the washing capdoes not leak to the outside.

The cartridge tank is filled with a washing liquid similar to thatprepared in the washing bath 20.

According to such a washing apparatus, the washing liquid is dischargedfrom the main body 1 into the washing cap through the discharge pipe 2,whereby the head can be washed. The discharged washing liquid istransferred from the washing cap into the filter unit 5 through thesuction pipe 4. After removing any loose hair, dandruff and the likethrough the filter unit 5, the washing liquid is collected in thecartridge tank.

The washing liquid collected in the cartridge tank is pumped into thescrew pump 12 in the same manner as for the washing apparatus used inthe method of washing the dog. In the screw pump 12, air is dissolved inthe washing liquid, and the washing liquid is transferred from thegas-liquid separator 15 to the washing cap through the discharge pipe 2.

The nozzle unit 7 similar to that used in the washing apparatus used inthe method of washing the dog is provided in the connection portionbetween the discharge pipe 2 and the washing cap.

The washing liquid is provided with a large number of microbubbles whenpassing through the nozzle unit 7, dispersed in a number of directions,and discharged into the washing cap.

When washing hair using the washing apparatus according to the presentinvention, the hair and the scalp are not damaged since it isunnecessary to directly scrub the hair and the scalp. Moreover, wastesinside the pores can be reliably removed in a short period of time.

After the completion of washing, the washing cap is removed from thehead, and the washing agent is removed by rinsing the head with waterfrom a shower nozzle or the like.

The washing apparatus according to the present invention can suppressdandruff, itching, and fallen hair without damaging the scalp and hair,and can safely wash the hair of a person suffering from a scalp diseaseor a person having a sensitive skin (e.g. the scalp gets rough or anallergy occurs when using a known washing agent).

The present invention is not limited to the above embodiments. Variousmodifications may be made without departing from the scope of thepresent invention.

The washing apparatus according to the present invention can safely andeasily wash a human body (particularly hair, skin, and foot bottom) or apet, and keep the skin clean for a long period of time.

Since the washing apparatus according to the present invention isconvenient and safe and exhibits an excellent washing effect, thewashing apparatus according to the present invention may be utilized towash a human or a pet in the home; to wash the human body, hair, footbottom, and the like in a public bath, a hospital, a nursing home, anursing-care facility, and the like; to wash the human hair, footbottom, and the like in a barbershop, a beauty salon and the like; towash a pet in an animal hospital, an animal hotel, and the like; and thelike.

1. A method of treating a skin disease, a scalp disease or sensitiveskin, which comprises: providing a liquid washing composition comprisingat least one protease, at least one lipase and at least one alkali metalsalt, adding microbubbles to the liquid composition to form amicrobubble solution or adding the liquid composition into a microbubbleapparatus to introduce microbubbles into the liquid composition to forma microbubble solution, and washing the skin of a subject in needthereof with an effective amount of the microbubble solution fortreating skin disease, scalp disease or sensitive skin.
 2. The methodaccording to claim 1, wherein the liquid washing composition consistsessentially of at least one protease, at least one lipase and at leastone alkali metal salt.
 3. The method according to claim 1, wherein theat least one protease is present in an amount of 0.01 to 0.5 wt % basedon the total amount of the composition, and the at least one lipase ispresent in an amount of 0.1 to 1.0 wt % based on the total amount of thecomposition.
 4. The method according to claim 1, wherein the at leastone alkali metal salt is present in an amount of 80 to 99.5 wt % basedon the total amount of the composition.
 5. The method according to claim1, wherein the washing liquid composition further comprises at least onesilica-based desiccant.
 6. The method according to claim 1, wherein ascalp of a human body or fur of an animal is washed on said human bodyor animal.
 7. The method according to claim 1, wherein the at least onealkali metal salt is at least one compound selected from the groupconsisting of an alkali metal carbonate, an alkali metal hydrogencarbonate, an alkali metal sulfate, an alkali halide, and an alkalimetal salt of boron.
 8. The method according to claim 1, which furthercomprises filtering out contaminants released from the subject duringthe washing step.
 9. The method according to claim 1, wherein themicrobubbles have a diameter of 50 μm or less.
 10. The method accordingto claim 1, wherein the microbubbles are negatively charged.
 11. Amethod of suppressing hair loss, which comprises: providing a liquidwashing composition comprising at least one protease, at least onelipase and at least one alkali metal salt, adding microbubbles to theliquid composition to form a microbubble solution or adding the liquidcomposition into a microbubble apparatus to introduce microbubbles intothe liquid composition to form a microbubble solution, and washing theskin of a subject in need thereof with an effective amount of themicrobubble solution for suppressing hair loss.
 12. The method accordingto claim 11, wherein the washing composition consists essentially of atleast one protease, at least one lipase and at least one alkali metalsalt.
 13. The method according to claim 11, wherein the at least oneprotease is present in an amount of 0.01 to 0.5 wt % based on the totalamount of the composition, and the at least one lipase is present in anamount of 0.1 to 1.0 wt % based on the total amount of the composition.14. The method according to claim 11, wherein the at least one alkalimetal salt is present in an amount of 80 to 99.5 wt % based on the totalamount of the composition.
 15. The method according to claim 11, whereinthe washing liquid composition further comprises at least onesilica-based desiccant.
 16. The method according to claim 11, whereinthe at least one alkali metal salt is at least one compound selectedfrom the group consisting of an alkali metal carbonate, an alkali metalhydrogen carbonate, an alkali metal sulfate, an alkali halide, and analkali metal salt of boron.
 17. The method according to claim 11, whichfurther comprises filtering out contaminants released from the subjectduring the washing step.
 18. The method according to claim 11, whereinthe microbubbles have a diameter of 50 μm or less.
 19. The methodaccording to claim 11, wherein the microbubbles are negatively charged.